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Monday, February 7, 2011

Microscopes

Use a microscope to:

  • study chemical reactions
  • how a cell works
  • study cells
  • see organelles
  • see DNA replicate
Insulin is used to re-harvest genes. Sophisticated microscopes are used.

Biopsy: a sample of a tumor looked at under a microscope.
Tissues: multiple cells put together.

Oil immersion lens is used specifically to look at a specimen with a drop of oil on it to give a very high resolution magnification. Field of view changes with your change of magnification.

You can move the stage up, down and sideways. 
The diaphragm controls the amount of light. Only use lens paper to clean the lens.

Tissues put together make organs, organs put together make systems. Skin is the largest organ, second largest is the liver.

Chapter One: Cell Biology

1938, James Hillier and Albert Prebus at U of T produced the first functional electron microscope. Scientists able to view viruses and sub cellular parts with great resolution. Viruses are measured in microbes.

Resin: solidified cube of a cell.

The three types of Microscopes:
1) Transmission Electron Microscope (TEM)
  • Beam of electron passes through very thin slices of specimen
  • Two dimensional view
  • Time consuming to prepare specimen
  • View dead specimen only
  • Not portable
2) Scanning Electron Microscope (SEM)
  • Electron scans across the surface of the sample
  • Secondary electron releases by the sample to form a 3-D image
3) Scanning Tunneling Microscope (STM)
  • A minuscule electron probe is placed near the sample
  • The created image is converted to a topographic image by the computer
  • Can manipulate molecules and atoms
  • Study biology at the atomic level

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